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[目的]探讨二硫化碳(CS2)对雄性大鼠下丘脑-垂体-性腺轴超微结构的影响及一氧化氮(NO)的干预作用。[方法]将24只雄性SD大鼠按体重随机分为4组:对照组、CS2染毒组、硝普钠(sodium nitroprusside,SNP)干预组和N-甲基-L-精氨酸(L-NMMA)干预组。除对照组外,其余3组均以1250mg/m3CS2进行静式吸入染毒,2h/d,5d/周,共10周,SNP和L-NMMA干预组在动物染毒结束前10d开始分别腹腔注射SNP(5mg/kg)和L-NMMA(2mg/kg),1次/d。染毒结束后,取下丘脑、垂体和睾丸组织,利用透射电子显微镜观察组织超微结构的改变。[结果]CS2染毒可造成下丘脑神经元、垂体促性腺激素细胞、生长激素细胞和睾丸支持细胞线粒体肿胀,内质网扩张,SNP对CS2引起的下丘脑、垂体、睾丸组织的损伤具有拮抗作用,而L-NMMA则进一步导致病变的发生。[结论]CS2可造成下丘脑、垂体和睾丸组织超微结构的改变,NO在此过程中发挥重要作用。
[Objective] To investigate the effects of carbon disulfide (CS2) on the ultrastructure of hypothalamus-pituitary-gonad axis in male rats and the intervention of nitric oxide (NO). [Methods] Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups according to body weight: control group, CS2-treated group, sodium nitroprusside (SNP) intervention group and N-methyl-L-arginine -NMMA) intervention group. Except the control group, the other three groups were treated with inhalation of 1250mg / m3CS2 for 2h / d, 5d / week for 10 weeks. SNP and L-NMMA intervention groups were injected intraperitoneally SNP (5 mg / kg) and L-NMMA (2 mg / kg) once daily. After the exposure, the thalamus, pituitary and testis were removed and the ultrastructural changes of the tissue were observed by transmission electron microscopy. [Result] CS2 exposure induced mitochondrial swelling and endoplasmic reticulum dilation in hypothalamic neurons, pituitary gonadotrophs, somatotrophs and testicular supporting cells. SNP antagonized the injury of hypothalamus, pituitary and testis induced by CS2 Role, while L-NMMA is further lead to the occurrence of lesions. [Conclusion] CS2 can cause ultrastructural changes of hypothalamus, pituitary and testis tissue, and NO plays an important role in this process.