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目的探讨不同核酸检测方法在非淋球菌性尿道炎病原体检测中的临床价值。方法对173例疑似非淋球菌性尿道炎感染患者尿液以及尿道分泌物样本运用RNA恒温扩增技术(RNA-SAT)和实时荧光定量PCR技术(FQ-PCR)分别进行解脲支原体(Uu)和沙眼衣原体(Ct)检测,并对结果统计分析。结果 2种方法检测,尿道分泌物样本的Uu阳性率均高于尿液样本,差异有统计学意义(χ~2=4.011,P<0.05),而Ct阳性率经χ~2检验比较差异无统计学意义(χ~2=0.25,P>0.05)。Uu-尿液检测的敏感度(100.0%)高于Uu-尿道拭子(88.0%),差异有统计学意义(χ~2=9.049,P<0.01)。Ct-尿液和Ct-尿道拭子,二者敏感度差异无统计学意义(χ~2=0.642,P>0.05)。结论 RNA-SAT法既具备了FQ-PCR的高敏感度和特异度,又能很好地判断预后效果,同时可采用尿液作为待检样本,具有取样方便、耗时短、污染小及结果准确等优点,可用于临床实验室检测与疗效监测。
Objective To investigate the clinical value of different nucleic acid detection methods in the detection of non-gonococcal urethritis pathogens. Methods Totally 173 samples of urinary and urethral secretions from patients with suspected non-gonococcal urethritis infection were subjected to Uu-Uu (RNAi) and real-time fluorescence quantitative PCR (FQ-PCR) And Chlamydia trachomatis (Ct) detection, and statistical analysis of the results. Results The positive rate of Uu in urine samples was higher than that in urine samples by two methods (χ ~ 2 = 4.011, P <0.05), while the positive rate of Ct was no significant difference by χ ~ 2 test Statistical significance (χ ~ 2 = 0.25, P> 0.05). The sensitivity of Uu-urine test (100.0%) was higher than that of Uu-urethral swabs (88.0%), with a statistically significant difference (χ ~ 2 = 9.049, P <0.01). There was no significant difference in sensitivity between Ct-urine and Ct-urethral swab (χ ~ 2 = 0.642, P> 0.05). Conclusion The RNA-SAT method not only possesses the high sensitivity and specificity of FQ-PCR, but also can judge the prognosis well. At the same time, urine can be used as sample to be tested, which has the advantages of convenient sampling, short time-consuming, less pollution and result Accurate and other advantages, can be used for clinical laboratory testing and monitoring of efficacy.