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目的:探讨不同禁欲时间采集精液对体外受精-胚胎移植(IVF-ET)实验室指标及临床结局的影响。方法:选取2011年6月~2013年7月在该院生殖中心行IVF-ET治疗符合纳入标准的160例患者(160个周期)为研究对象,根据男方禁欲时间不同随机分为:2~3天组、4~5天组、6~7天组及大于7天组4组,每组40例(40个周期)。比较4组患者洗精前后精液质量、MⅡ卵受精率、正常受精率、正常卵裂率、可用胚胎率、优质胚胎率、临床妊娠率、着床率及早期流产率是否有差异。结果:1随禁欲时间延长4组患者的精液量、精子密度上升(P<0.05);精子活率、前向运动精子率及正常形态率逐渐下降(P<0.05)。而精液经优选技术处理后,以上各指标均有明显改善,处理前后比较差异有统计学意义(P<0.05)。2 4组患者的MⅡ卵受精率、正常受精率、正常卵裂率、可用胚胎率、优质胚胎率、临床妊娠率、着床率及早期流产率方面比较,差异均无统计学意义(P>0.05)。结论:IVF-ET中精液质量正常的患者不同禁欲时间采集的精液对实验室指标及临床结局无明显影响。
Objective: To investigate the effects of different time of abstinence-collected semen on laboratory indexes and clinical outcome of IVF-ET. METHODS: A total of 160 patients (160 cycles) eligible for IVF-ET at the reproductive center of our hospital from June 2011 to July 2013 were enrolled in this study. Patients were randomly divided into 2 ~ 3 Day group, 4 ~ 5 days group, 6 ~ 7 days group and more than 7 days group 4 groups, 40 cases (40 cycles). The differences of semen quality, M Ⅱ egg fertilization rate, normal fertilization rate, normal cleavage rate, available embryo rate, high quality embryo rate, clinical pregnancy rate, implantation rate and early miscarriage rate were compared before and after washing. Results: 1 The semen volume and sperm density increased in 4 groups of patients with prolonged abstinence time (P <0.05). The sperm motility, motile sperm motility and normal morphology decreased gradually (P <0.05). The semen by the preferred technology, the above indicators were significantly improved, before and after treatment was statistically significant difference (P <0.05). There were no significant differences in M Ⅱ egg fertilization rate, normal fertilization rate, normal cleavage rate, available embryo rate, high quality embryo rate, clinical pregnancy rate, implantation rate and early miscarriage rate between 24 groups (P> 0.05). CONCLUSIONS: Semen collected at different abstinence times in patients with normal sperm mass in IVF-ET had no significant effect on laboratory parameters and clinical outcomes.