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为了建立HCV体外复制系统,作者测定了不同细胞系对HCV的易感性。采用日本红十字会血液中心提供的1090号血清感染多种细胞系,该血清中HCV浓度高于10~6拷贝/ml,所用的细胞系包括人肝细胞癌细胞系HuH7,HLE,HLF,PLC/PRF/5,Li21,Li22,Li23,Li24;胆管癌细胞系HuCCT1,HuH28;人单核细胞白血病细胞系U937,THP-1;猪仔细胞系PK15(该细胞系已成功用于黄病毒培养)和HTLV-I病毒感染的细胞系MT-2,C91/PL及COH等。
In order to establish a HCV in vitro replication system, the authors determined the susceptibility of different cell lines to HCV. A variety of cell lines were infected with 1090 Serum provided by the Japan Red Cross Blood Center with HCV concentrations higher than 10 to 6 copies / ml. The cell lines used included human hepatocellular carcinoma cell lines HuH7, HLE, HLF, PLC / PRF / 5, Li21, Li22, Li23 and Li24; cholangiocarcinoma cell lines HuCCT1 and HuH28; human monocytic leukemia cell line U937 and THP-1; piglet cell line PK15 (this cell line has been successfully used in the culture of flavivirus ) And HTLV-I virus-infected cell lines MT-2, C91 / PL and COH.