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目的探讨CD70基因在肝癌中的表达及其在肝癌免疫逃逸中的作用。方法采用RT-PCR检测CD70在肝癌细胞系和肝癌组织的表达;构建CD70真核表达载体,瞬时转染HepG2细胞,与Jurkat细胞共培养,通过细胞增殖试剂(CCK8)检测对其淋巴细胞增殖的抑制作用。结果①肝癌细胞系BEL-7402、SMMC-7721中CD70表达呈阳性,HepG2细胞中CD70表达呈阴性;10例肝癌组织中3例CD70表达呈阳性,2例癌旁组织CD70表达均呈阴性;②成功构建CD70的真核表达载体,瞬时转染HepG2细胞,与活化的Jurkat细胞共培养。转染CD70后的HepG2与Jurkat共培养后,Jurkat增殖抑制率(65.03%)与空细胞对照组和空载体对照组(38.01%、30.15%)相比显著升高(P<0.01)。结论CD70基因在肝癌中呈阳性表达,并可抑制淋巴细胞的增殖活性,在肝癌细胞免疫逃逸中起了重要作用。
Objective To investigate the expression of CD70 gene in hepatocellular carcinoma (HCC) and its role in immune escape of HCC. Methods The expression of CD70 in hepatocellular carcinoma and hepatocellular carcinoma was detected by RT-PCR. CD70 eukaryotic expression vector was constructed and transfected into HepG2 cells transiently. The cells were co-cultured with Jurkat cells. The proliferation of lymphocytes was detected by cell proliferation assay (CCK8) Inhibition. Results ① The expression of CD70 in BEL-7402 and SMMC-7721 cells was positive, but CD70 was negative in HepG2 cells. CD70 expression was positive in 3 of 10 HCC tissues and negative in 2 adjacent tissues. The eukaryotic expression vector of CD70 was successfully constructed and transiently transfected into HepG2 cells for co-culture with activated Jurkat cells. After co-culturing HepG2 with CD70, the proliferation inhibition rate of Jurkat (65.03%) was significantly higher than that of blank control group and empty vector control group (38.01%, 30.15%) (P <0.01). Conclusion CD70 gene is positively expressed in hepatocellular carcinoma and can inhibit the proliferation activity of lymphocytes, which play an important role in the immune escape of hepatocellular carcinoma.