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目的:优选抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)的提取工艺。方法:采用紫外-可见分光光度法测定乙醇提取物中皂苷、黄酮、木质素类成分含量;正交试验设计优选抗柯萨奇B病毒性心肌炎胶囊的提取工艺。结果:乙醇提取最佳工艺为10倍量70%乙醇加热回流2次,每次1.5 h。结论:优选的提取工艺稳定,安全,简便易行。
Objective: To optimize the extraction process of anti-Coxsackie B virus myocarditis capsule (K-CoxB-JN). Methods: The contents of saponin, flavonoids and lignin in ethanol extract were determined by UV-Vis spectrophotometry. The extraction process of anti-Coxsackie B virus myocarditis capsules was optimized by orthogonal design. Results: The best ethanol extraction process was 10 times the amount of 70% ethanol heated to reflux twice, each 1.5 h. Conclusion: The optimal extraction process is stable, safe and easy to operate.