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目的:探讨贵州省汉族人群载脂蛋白M(apoM)基因rs707922位点多态性与冠心病(CHD)的相关性。方法:(1)采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测冠心病组(220例)和对照组(非冠心病患者233例)apoM基因rs707922位点的多态性。(2)自动生化仪检测血清中甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平。(3)应用人apoM ELISA试剂盒测定血清apo M。结果:(1)apo M基因rs707922位点三种基因型GG、GT、TT在冠心病组和对照组间差异有统计学意义(χ~2=6.408,P<0.05)。冠心病组T等位基因的频率显著高于健康对照组差异有统计学意义(χ~2=5.430,P<0.05)。(2)冠心病组及健康对照组GT+TT基因型的血清apo M水平和高密度脂蛋白胆固醇水平显著低于GG基因型人群,GT+TT基因型的血清总胆固醇水平明显高于GG基因型的人群。结论:apoM基因rs707922位点等位基因T可能是冠心病的一个遗传危险因子。
Objective: To investigate the association of rs707922 polymorphism of apolipoprotein M (apoM) gene with coronary heart disease (CHD) in Han nationality in Guizhou province. Methods: (1) PCR-RFLP was used to detect rs707922 apoM gene polymorphism in coronary heart disease (220 cases) and control group (233 cases without coronary heart disease) State. (2) The levels of triglyceride (TG), cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum were detected by automatic biochemical analyzer. (3) Serum apo M was measured using human apoM ELISA kit. Results: (1) There were significant differences in GG, GT and TT genotypes between rs707922 apo M genotype and control group (χ ~ 2 = 6.408, P <0.05). The frequency of T allele in CHD group was significantly higher than that in healthy control group (χ ~ 2 = 5.430, P <0.05). (2) The levels of apo M and HDL-C of GT + TT genotype in CHD group and healthy control group were significantly lower than those in GG genotype, and the serum total cholesterol level of GT + TT genotype was significantly higher than that of GG gene Type of people. Conclusion: The allele T rs707922 of apoM gene may be a genetic risk factor for coronary heart disease.