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本文探讨了淹水条件下的温带、亚热带和热带的11种土壤中,微生物分解大家熟知的残留性有机氯杀虫剂γ—六六六的状况,结果表明在供试的11种土壤中,除3种土壤外,培养70天,大部分土壤中的γ—六六六消失了。土壤中的有机物含量、γ—六六六分解菌数与γ—六六六的分解率之间看不到有意义的相关性。在没有发现γ—六六六分解的3种土壤中,一般地说,有机物含量、γ—六六六分解数以及pH都有低的倾向。培养温度和γ—六六六分解率的关系为,在20℃、30℃、40℃时,温度越高,分解率越高,特别是印度尼西亚的Seputih土壤有较高的分解率。从各土壤中分离的γ—六六六分解菌是嫌气性的梭状芽孢杆菌,即使在培养基的分解试验中,40℃时的分解活性最高,分解时的中间未知化合物量也在40℃时最高。根据气相色谱—质谱分析,由分离菌生成的中间未知化合物断定为γ—四氯环已烯。
In this paper, the status of residual organic organophosphate insecticide γ-BHC, which is well known by microorganisms, was investigated in eleven soils of temperate zone, subtropical zone and tropical zone under flooding condition. The results showed that in the 11 kinds of soils tested, In addition to the three kinds of soil, the cultivation of 70 days, most of the soil γ-666 disappeared. There was no significant correlation between the content of organic compounds in the soil, the fraction of γ-HCH and the decomposition rate of γ-BHC. In the three kinds of soils that did not find the decomposition of γ-HCH, generally speaking, the organic content, γ-HCH decomposition number and pH tended to be low. The relationship between culture temperature and γ-HCH fraction is that at 20 ° C, 30 ° C and 40 ° C, the higher the temperature, the higher the decomposition rate. In particular, Seputih soil in Indonesia has a higher decomposition rate. The γ-HCH isolates from each soil are anaerobic Clostridium, and have the highest decomposition activity at 40 ° C even in the decomposition test of the medium, and the amount of intermediate unknown compounds during decomposition is also about 40 ℃ when the highest. According to gas chromatography-mass spectrometry analysis, the intermediate unknown compound formed by the isolated bacteria was identified as γ-tetrachlorocyclohexene.