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为探讨臭氧(O3)是否伤害叶片远轴侧以及对与近轴侧和远轴侧伤害的异同,以玉簪为材料通过显微观察、叶绿素荧光诱导动力学和气体交换等技术研究了该问题。叶片形态和显微观察表明,200μg·kg-1 O3处理10 d后玉簪叶片近轴侧表面和叶肉组织呈现明显伤害症状,而远轴侧在300μg·kg-1 O3条件下也发生明显伤害。O3处理显著改变了叶片近轴侧和远轴侧荧光诱导动力学曲线的形状;尽管两侧荧光诱导动力学曲线的K、J和I点相对荧光产量均增加,但仅近轴侧K点的相对荧光上升幅度更加明显。此外,O3处理下玉簪叶片的光合速率和叶绿素含量均下降,相对电导率和膜脂过氧化程度大幅增加。鉴于显微观察和荧光诱导动力学的测定结果,我们认为O3能够同时伤害玉簪叶片近轴侧和远轴侧叶肉组织,并就臭氧对玉簪叶片两侧的伤害机制进行了比较和讨论。
In order to investigate whether ozone (O3) harms the distal and distal paraxial sides of the leaves, this paper investigates the effects of O3 on the paraxial side and the distal side by microscopy, chlorophyll fluorescence-induced kinetics and gas exchange. Morphology and microscopic observation of leaves showed that the injury of symptoms appeared on the paraxial side of leaves and mesophyll tissue of leaves of Hosta planta at 200 μg · kg -1 O 3 treatment for 10 d, while the damage on the distal axis was also observed under the condition of 300 μg · kg -1 O 3. O3 treatment significantly changed the shape of the fluorescence induced kinetic curve on the paraxial side and distal side of the leaf. Although the relative fluorescence yields increased at K, J and I points on both sides of the fluorescence-induced kinetic curve, Relative fluorescence increased more significantly. In addition, photosynthetic rate and chlorophyll content of Hosta were decreased under O3 treatment, and relative conductivity and membrane lipid peroxidation increased significantly. In view of the results of microscopic observation and fluorescence-induced kinetics, we think that O3 can damage both mesophyll and distal mesophyll tissues of Hosta hosta leaves at the same time, and the damage mechanism of ozone on both sides of Hosta was compared and discussed.