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目的探讨急性期升高血压治疗对局灶性脑缺血大鼠脑梗塞体积和脑血流的影响。方法健康成年雄性SD大鼠36只,被随机分为单纯缺血3、4、6h组及缺血2、3、5h升高血压1h组,共6组,每组6只。制备大脑中动脉梗死模型,采用激光多普勒血流仪测量大鼠局灶性脑缺血各时相皮层缺血中心边缘区局部脑血流(rCBF),并于各时间点取大鼠脑切片,红四氮唑染色后,计算机图像分析系统测量脑梗死体积。结果缺血2、3、5h升压组大鼠rCBF分别恢复至基础rCBF的(81.8±3.1)%、(56.0±2.1)%和(38.8±2.0)%。缺血2h、3h升压组脑梗死体积分别为(14±7)mm3、(90±24)mm3,均显著小于相应的单纯缺血组比较梗塞体积显著缩小(均P<0.05)。结论改善缺血中心边缘区脑血流可能是急性期升压疗法治疗局灶性脑缺血损伤的主要机制之一。
Objective To investigate the effects of acute hypertension on cerebral infarction volume and cerebral blood flow in focal cerebral ischemia rats. Methods Thirty-six healthy adult male Sprague-Dawley rats were randomly divided into three groups: Ischemia 3, 4 and 6 hours and Ischemia 2 hours, 3 hours and 5 hours respectively. There were 6 rats in each group. The middle cerebral artery occlusion (MCAO) model was prepared and the regional cerebral blood flow (rCBF) was measured by laser Doppler flowmetry. The regional cerebral blood flow (rCBF) After sectioning and staining with red tetrazolium, a computerized image analysis system was used to measure the infarct volume. Results The rCBF increased to (81.8 ± 3.1)%, (56.0 ± 2.1)% and (38.8 ± 2.0)%, respectively, in basal rCBF in 2 h, 3 h and 5 h hypertensive rats. The volume of cerebral infarction was (14 ± 7) mm3 and (90 ± 24) mm3 respectively in 2 h and 3 h ischemia group, which were significantly smaller than those in the corresponding ischemia group (all P <0.05). Conclusion Improving the cerebral blood flow in the peripheral region of the ischemic center may be one of the main mechanisms in the treatment of focal cerebral ischemia injury in acute phase.