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1964年Epstein和Barr首先报告从Burkitt淋巴瘤活检组织成功地培养了淋巴样细胞。其后不久,Iwakata等从慢性骨髓性白血病人的血建立了淋巴样细胞系;Moore等从两个正常人血建立了淋巴样细胞系。据报道目前世界各地从各种不同来源建立的这类细胞系已有数千个之多。国内这方面的工作也有所开展,如中国医学科学院肿瘤防治研究所等自鼻咽癌活检组织和扁桃体组织建立了11个淋巴母细胞样细胞系;中国医学科学院输血血液病研究所从两个粒单型白血病病人外周血建立了两个细胞系。我院自1976年开始以来从健康人外周血亦先后建立了10个淋巴样细胞系。
In 1964, Epstein and Barr first reported successfully culturing lymphoid cells from Burkitt’s lymphoma biopsies. Shortly thereafter, Iwakata et al. Established a lymphoid cell line from human myelogenous leukemia; Moore et al. Established a lymphoid lineage from two normal human blood. Thousands of such cell lines have been reported from various sources around the world at present. China has also carried out this work, such as the Chinese Academy of Medical Sciences Institute of Oncology and other nasopharyngeal carcinoma biopsy tissue and tonsil tissue established 11 lymphoblastoid cell line; Chinese Academy of Medical Sciences Institute of transfusion blood from two tablets Peripheral blood mononuclear leukemia patients established two cell lines. Since 1976, our hospital has also established 10 lymphoid cell lines from healthy human peripheral blood.