甘蓝型油菜硫苷组分的双标图分析

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采用GGE双标图法,对11份硫苷组分不同甘蓝型油菜进行4×7不完全双列杂交试验,旨在对各杂交组合的硫苷组分进行分析,从而筛选出其配合力较低的亲本组合来满足降低后代硫苷质量摩尔浓度的要求.结果显示:1对于2-羟-3-丁烯基而言,亲本一般配合力(GCA)较低的是父本D21R,D1R和母本D3AB,特殊配合力(SCA)较低的是父本91R,56R和母本D3AB,SCA较低的组合有S3×R3,S3×R7和S1×R3等;2对于3-丁烯基而言,亲本GCA较低的是父本15R,91R和母本Gd1AB,D3AB,SCA较低的是父本D1R,D21R和母本D3AB,SCA较低的组合有S3×R3,S3×R7和S1×R7等;3对父本GCA而言,3-丁烯基、4-羟-3-吲哚甲基、苯乙基、4-戊烯基与2-羟-3-丁烯基呈正相关,苯乙基、4-戊烯基与4-羟-3-吲哚甲基呈负相关.对母本GCA而言,3-丁烯基、4-戊烯基与2-羟-3-丁烯基呈正相关,苯乙基、4-羟-3-吲哚甲基与2-羟-3-丁烯基呈负相关;4各杂交组合硫苷组分的表型关系中,亲本针对各组分GCA的相关性与母本配合力效应双标图中所显示出来的结果基本一致.利用GGE双标图分析表明,选用D3AB作母本、D21R及D1R作为父本有利于降低杂种后代油菜籽中硫代葡糖苷的质量摩尔浓度. In this study, 4 × 7 incomplete diallel cross tests were performed on 11 Brassica napus cultivars with the GGE double-labeled map method. The aim of this study was to analyze the glucosinolate components of the cross combinations, Low parental combinations to meet the requirement of reducing the molality of the descended hawthorn.The results showed that: 1 for the 2-hydroxy-3-butenyl, the lower GCA was the male parent D21R, D1R and D3AB, S3A, S3R7 and S1R3 in the lower SCA were lower for the male parent D3AB and lower SCA than for the male parent 91R and 56R and the female parent D3AB. 2 For 3-butenyl In the lower GCA, the parental 15R and 91R and the female parent Gd1AB and D3AB were lower, while the lower parental D1R and D21R and the female parent D3AB. The lower combination of SCA was S3 × R3 and S3 × R7 and S1 × R7, etc .; 3 pairs of maleic GCA, 3-butenyl, 4-hydroxy-3-indolylmethyl, phenethyl, 4-pentenyl and 2-hydroxy- Phenethyl and 4-pentenyl were negatively correlated with 4-hydroxy-3-indolylmethyl.For female GCA, 3-butenyl, 4-pentenyl and 2-hydroxy-3 -butenyl was positively correlated, Phenethyl, 4-hydroxy-3-indolylmethyl and 2-hydroxy-3-butenyl was negatively correlated; 4 The phenotypes of glucosinolate components , The parental GCA for each component correlation with the maternal combining ability effect double marked the results shown are basically the same.Using the GGE double plot analysis showed that the choice of D3AB as the female parent, D21R and D1R as a parent benefit Reduce the mass concentration of thioglucoside in the hybrid progeny rapeseed.
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