The samples of ductile-rheologic deformational augen granite from the Yunkai uplift area, western Guangdong province, were determined by the whole-rock Sm-Nd, Pb-Pb and Rb-Sr isotopic dating to have an Sm-Nd isochron age of 1414± 68 Ma, a Pb-Pb isochron age of 1388± 90 Ma and a Rb-Sr isochron age of 490± 36 Ma. The first two ages are interpreted as the formation age of this suite of granite and the last age represents the timing of the tectono-thermal event of Caledonian ductile-rheologic shear partial melting. It is indicated that in the study area not only an orogeny took place in the Caledonian, but also a more important tectono-magmatic activity occurred in the Mesoproterozoic there , which may be related to the subduction-collision between the Yangtze block and Cathaysia block.