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牙体硬组织的生理状况、牙菌斑和唾液中的矿物质成份都与龋病的发生有着密切的关系。为了研究患龋时牙菌斑和唾液中矿物质结构变化,作者选择了在一年中处于同一劳动环境、日常生活和营养状况的18岁～20岁健康男性青年20名,其中10名牙列完整、无龋齿,另10名牙列完整,分别患有1～5个初期龋(无龋齿合并症,亦未作充填治疗)。在获取标本前5天被检者停止口腔清洁护理,于第6天清晨空腹时采集标本。用特制的菌斑采集工具取样,
Tooth hard tissue physiological conditions, plaque and saliva in the mineral composition are closely related with the occurrence of dental caries. In order to study the changes of mineral structure in dental plaque and saliva with caries, the author chose 20 healthy males aged 18-20 years in the same work environment, daily life and nutritional status in a year, of which 10 dentition Complete, tooth-free, and the other 10 dentition intact, suffering from 1 to 5 primary caries (no dental caries complications, nor filling treatment). The subjects stopped oral cleaning and nursing five days before the specimens were taken and samples were collected on the morning of the sixth day in fasting. With a special plaque collection tool sampling,