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目的:分析分枝杆菌细胞壁的组成及特性。方法:将制备好的样本放在生物安全操作柜中,静置30 min,使样本在奈米银粒子数组(SERS)基板上干燥,测量SERS讯号。比较结核分枝杆菌复合群(MTBC)与非结核分枝杆菌(NTM)的Sup1的SERS讯号。结果:生长在不同培养基下,所取得的M.tuberculosis的上清液光谱的主要拉曼位移是一致的。结论:SERS技术确实拥有区别结核菌群和非结核分枝杆菌的能力。但若要应用在临床鉴定,需要测试更多的临床分枝杆菌,以了解此方法的敏感度与特异度为多少,藉此评估此方法的应用价值和可行性。
Objective: To analyze the composition and characteristics of mycobacteria cell wall. Methods: The prepared samples were placed in a biosafety cabinet and allowed to stand for 30 min. The samples were dried on a SERS substrate to measure the SERS signal. The SERS signal was compared between Mycobacterium tuberculosis complex (MTBC) and non-tuberculosis (NTM) Sup1. Results: The main Raman shifts of the supernatant spectra of M. tuberculosis grown in different media were consistent. Conclusion: SERS technology does have the ability to distinguish between tuberculosis and non-tuberculous mycobacteria. However, if it is applied in clinical identification, more clinical mycobacteria should be tested to know the sensitivity and specificity of this method, so as to evaluate the application value and feasibility of this method.