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目的探讨胃癌细胞及组织中丝氨酸／苏氨酸激酶15(STK15)mRNA和蛋白质表达与临床病理指标、胃癌细胞增殖的关系。方法采用实时定量PCR及Western blot分别检测正常胃黏膜上皮细胞株GES-1、胃癌细胞株SUN-16、KATOIII、MKN45、AGS、N87及60份新鲜切除的胃癌组织中 STK15基因mRNA及蛋白质表达。RNA干扰技术(RNAi)抑制MKN45、AGS、N87细胞株STK15表达,流式细胞仪检测细胞周期变化,MTT法检测细胞增殖速率变化,数据行秩和检验及t检验。结果 SUN-16、KATOⅢ、MKN45、AGS、N87中STK15基因mRNA及蛋白质表达均明显高于GES-1;60份胃癌组织中STK15 mRNA及蛋白质表达水平中位值分别为3．175×10-3与1．261,明显高于正常组织的0．532×10-3与0．224(P<0．05);胃癌组织中STK15 mRNA水平与胃癌分化及浸润深度有关(P< 0．05);而蛋白质表达水平与胃癌分化有关(P<0．05)。抑制STK15表达后,MKN45、AGS、N87细胞株 G2期DNA含量细胞分别为(26．1±6．1)％、(25．6±7．9)％及(20．6±6．1)％,明显高于对照组的(12．5 ±4．9)％、(10．9±4．4)％及(8．7±3．5)％(P<0．05);细胞增殖速率减慢(P<0．05)。结论 STK15 的过表达可能在胃癌发生发展中起促进作用,可作为胃癌某些生物学行为新的判定指标;STK15的过表达与胃癌细胞增殖有关。
Objective To investigate the relationship between the expression of serine / threonine kinase 15 (STK15) mRNA and protein, the clinicopathological parameters and the proliferation of gastric cancer cells in gastric cancer cells and tissues. Methods The expression of STK15 mRNA and protein in gastric cancer cell line GES-1, gastric cancer cell lines SUN-16, KATOIII, MKN45, AGS, N87 and 60 freshly resected gastric cancer tissues were detected by real-time PCR and Western blot respectively. The expression of STK15 in MKN45, AGS and N87 cell lines was inhibited by RNA interference (RNAi), the cell cycle was detected by flow cytometry, and the proliferation rate was detected by MTT assay. The data were analyzed by rank sum test and t test. Results The mRNA and protein expressions of STK15 in SUN-16, KATOⅢ, MKN45, AGS and N87 were significantly higher than those in GES-1. The median of STK15 mRNA and protein expression in 60 gastric cancer tissues was 3.175 × 10-3 And 1.261, significantly higher than that of normal tissues (P <0.05). The levels of STK15 mRNA in gastric cancer tissues were correlated with the differentiation and depth of invasion (P <0.05) ; While the protein expression level was related to the differentiation of gastric cancer (P <0.05). The inhibitory effect of STK15 on G2 DNA of MKN45, AGS and N87 cell lines was (26.1 ± 6.1)%, (25.6 ± 7.9)% and (20.6 ± 6.1) %, Which was significantly higher than that of the control group (12.5 ± 4.9)%, (10.9 ± 4.4)% and (8.7 ± 3.5)%, respectively The rate was slower (P <0.05). Conclusion Overexpression of STK15 may play an important role in the carcinogenesis and progression of gastric cancer. It may be used as a new indicator of some biological behaviors of gastric cancer. The overexpression of STK15 may be related to the proliferation of gastric cancer cells.