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为了解山东省正常人群破伤风抗体水平,评价既往破伤风类毒素(TT)免疫工作质量,我们每年均有计划地对部分人群开展破伤风抗体水平监测,现将1997年监测结果报告如下.1 材料与方法1.1 标本的采集 从综合监测点(县级)中随机抽取3个监测点,每个监测点随机抽取2个乡,按<2,2～,5～,8～,11～,15～,20～,40～岁8个年龄组,每组抽30人;每人采血约0.2ml,分离血清,置-20℃保存待检.1.2 试剂来源与检测方法 由中国药品生物制品检定所提供标准破伤风抗毒素(4 IU/ml)、破伤风抗体致敏血球、对照血球和实验用稀释液(批号97—10).按卫生部《计划免疫技术管理规程》要求做间接血凝试验(IHA).1.3 结果的判定以“++”的血凝滴度作为间接血凝反应终点,以血清中破伤风抗毒素≥0.00156 IU/ml判定为阳性(达到保护水平).待检血清抗毒素单位=待检血清终点稀释度×标准血清终点稀释滴度所含单位数,终点稀释度所含单位为0.0078IU/ml.
To understand the level of tetanus antibodies in the normal population of Shandong Province and to evaluate the quality of previous immunization against tetanus toxoid (TT), we conducted annual surveillance of tetanus antibody levels in some of our populations. The monitoring results for 1997 are reported below.1 Materials and methods 1.1 Collection of specimens Three monitoring points were randomly selected from the comprehensive monitoring points (county level), and 2 townships were randomly selected from each monitoring point. According to the statistics of <2,2 ~, 5 ~, 8 ~, 11 ~, 15 ~ 20 ~ 40 years old 8 age groups, each group pumping 30 people; each blood about 0.2ml, serum was separated and stored at -20 ℃ to be seized.1.2 Source of reagents and detection methods by the China Pharmaceutical and Biological Products Provide standard tetanus antitoxin (4 IU / ml), tetanus antibody sensitized blood cells, control blood cells and experimental diluent (batch number 97-10). According to the Ministry of Health, “Immunization Technology Regulations” to do an indirect hemagglutination test IHA) .1.3 Determination of the results The hemagglutination titer “++” as the indirect end point of hemagglutination, serum tetanus antitoxin ≥ 0.00156 IU / ml was judged to be positive (to reach the level of protection.) To be tested serum antitoxin unit = End-point serum to be seized × standard serum end-point dilutions contained Bits, contained in the endpoint dilution units 0.0078IU / ml.