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目的 诱导能模拟人类Alzheimer病 (AD)的大鼠病理模型 ,用于该病的病理机制和治疗药物的研究。方法 采用双侧海马内一次性注射 β 淀粉样多肽 2 5～ 35片段(Aβ2 5～ 35)诱导大鼠AD模型 ;行为测试采用跳台法、穿梭法和Morris水迷宫法 ;病理检测采用HE染色、刚果红染色和银染。结果 跳台法、穿梭法和Morris水迷宫法试验结果提示海马内注射Aβ2 5～ 35可引起大鼠主动和被动回避性反射及空间分辨力降低 ;病理检查发现皮质和海马神经元数量减少、退变 ,皮质下血管淀粉样变及脑内出现纤维蛋白丝状物。结论 海马内注射Aβ2 5～ 35诱导的大鼠学习记忆功能低下模型在功能和形态上类似于AD
Objective To induce pathological models of Alzheimer’s disease (AD) in humans and to study the pathological mechanism and therapeutic agents of this disease. Methods A rat model of AD was induced by intramuscular injection of β amyloid peptide 25 ~ 35 (Aβ2 5 ~ 35) in both bilateral hippocampus. The behavior test was performed by using the jumping method, the shuttle method and the Morris water maze method. The pathological examination was performed by HE staining, Congo red staining and silver staining. Results The results of jumping test, shuttle test and Morris water maze test indicated that the injection of Aβ2 5-35 in the hippocampus could cause the active and passive avoidance reflexes and decrease the spatial resolution of the rats. The number of cortical and hippocampal neurons decreased and degenerated , Subcortical vascular amyloidosis and fibrin filaments in the brain. Conclusion The hippocampal injection of Aβ2 5 ~ 35 induced learning and memory deficit model in function and morphology similar to AD