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本文报道在两相溶剂体系中利用木瓜蛋白酶催化合成Aspartame前体Z－APM的研究。实验结果表明，木瓜蛋白酶在水-正丁醇两相体系具有较好的催化活性，在底物比（Z－L－Asp：L－PheOMe）为1：1，温度37℃条件下连续反应72h，Z－APM的产率可达62．8％。
This paper reports the catalytic synthesis of Aspartame precursor Z-APM using papain in a two-phase solvent system. The experimental results showed that papain had good catalytic activity in the water-n-butanol two-phase system. The reaction was carried out continuously at a substrate ratio of 1: 1 (L-PheOMe) at 37 ℃ for 72h , Z-APM yield up to 62.8%.