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目的:探讨rSj26-Sj32融合蛋白用于慢性日本血吸虫病的诊断价值。方法:用rSj26-Sj32融合蛋白和日本血吸虫成虫粗抗原(SjAWA)作为包被抗原建立酶联免疫吸附试验(ELISA)、斑点酶联免疫吸附试验(Dot-ELISA)和dipstick三种方法检测慢性日本血吸虫病患者血清特异性IgG抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病和囊型棘球蚴病患者以及健康人血清作为对照。结果:rSj26-Sj32融合蛋白通过三种方法检测慢性日本血吸虫病的敏感性分别为95.00%、92.50%和92.50%;特异性分别为97.67%、95.35%和97.67%。结论:rSj26-Sj32融合蛋白可用于慢性日本血吸虫病的免疫诊断。
Objective: To investigate the diagnostic value of rSj26-Sj32 fusion protein in chronic schistosomiasis japonica. Methods: ELISA, Dot-ELISA and dipstick were used to detect the expression of IL-6 and IL-6 in Chronic Japan Schistosomiasis patients with serum-specific IgG antibodies, while clonorchiasis, Paragonimus westermani, alveolar echinococcosis and cysticercosis patients and healthy human serum as a control. Results: The sensitivity of rSj26-Sj32 fusion protein to detect chronic schistosomiasis japonica by three methods was 95.00%, 92.50% and 92.50% respectively; the specificity was 97.67%, 95.35% and 97.67% respectively. Conclusion: The rSj26-Sj32 fusion protein can be used for immunological diagnosis of chronic schistosomiasis japonica.