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本文研究了6 0 Coγ射线辐照对蜜环菌的生物学效应。结果发现 :1 0～ 80Gy低剂量辐照能提高蜜环菌的生活力 ,促进其生长 ,提高可溶性蛋白质含量 ,对细胞膜脂过氧化无明显影响 ,2 0Gy辐照效果最显著 ,菌丝体生长量较对照增重 3 1 2 0 % ,MDA含量较对照低 2 7 4% ;大于 80Gy辐照则随剂量增大生长受抑制 ,膜脂质过氧化程度加剧。1 0～ 80Gy辐照还提高了菌丝体中SOD活性 ,高于 80Gy则随剂量增加酶活性降低 ;POD活性随辐照剂量递增酶活性提高 ,与MDA含量呈正相关。6 0 Coγ射线辐照提高了蜜环菌中核酸含量 ,且随剂量增加而增加 ,较对照分别提高 3 1 %～ 2 84 3 %和2 40 %～ 1 61 41 %。 2 0Gy剂量辐照使DNA、RNA和可溶性蛋白质含量均提高 ,3者呈明显正相关 ;2 0 0Gy辐照使DNA、RNA含量提高而可溶性蛋白质含量却呈下降趋势 ,其相关系数分别为 -0 83 89和 -0 73 40。琼脂糖凝胶电泳表明 ,2 0Gy辐照对DNA链无明显影响 ,而 2 0 0Gy辐照则出现严重拖尾 ,表明DNA链有严重断裂。DNA断裂主要发生在辐照后 6h之内 ,以后能自动修复
In this paper, the biological effects of 60 Co γ-ray irradiation on Armillaria mellea were studied. The results showed that low doses of 10 ~ 80 Gy could improve the viability of Armillaria mellea, promote its growth and increase the content of soluble protein, but had no significant effect on lipid peroxidation, The weight gain was 3120% compared with the control, and the content of MDA was 274% lower than that of the control. When the dosage was more than 80Gy, the growth was inhibited and the lipid peroxidation was aggravated. 1 0 ~ 80Gy irradiation also increased the activity of SOD in mycelium, above 80Gy decreased with increasing doses of enzyme activity; POD activity increased with increasing dose of enzyme activity, and MDA content was positively correlated. 60 Coγ-ray irradiation increased the nucleic acid content of Armillaria mellea, and increased with increasing doses, which increased by 31.1% -284.3% and 2.40% -1.641%, respectively. 2 0Gy dose of DNA, RNA and soluble protein content were increased, 3 were positively correlated; 200Gy irradiation DNA and RNA content but the soluble protein content showed a downward trend, the correlation coefficients were -0 83 89 and -0 73 40. Agarose gel electrophoresis showed that 20 Gy had no significant effect on the DNA strand, but severe tailing occurred at the 200 Gy band, indicating a serious DNA strand break. DNA breaks mainly occurred within 6h after irradiation, and could be repaired automatically afterwards