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作者用戊二醛处理血清,血清IgG与白蛋白形成交联分子,其电泳迁移度稍大于白蛋白,可对IgG顺利地进行电泳免疫扩散定量。本法操作简单,快速,血清IgG最低检出量为3.15—6.30μg/ml,变异系数为5.71～4.91％。测定了53例正常人血清IgG含量为12.05±3.0mg/ml。
The author treated with glutaraldehyde serum, serum IgG and albumin to form cross-linked molecules, the electrophoretic mobility slightly larger than albumin, IgG can be carried out successfully electrophoresis immune diffusion quantitative. This method is simple and rapid, the minimum detectable amount of serum IgG is 3.15-6.30μg / ml, the coefficient of variation is 5.71 ~ 4.91%. The serum IgG levels of 53 normal individuals were determined to be 12.05 ± 3.0 mg / ml.