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目的:探讨不同浓度SO2污染对运动大鼠血清和肺组织白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平的影响,从细胞因子的角度揭示SO2污染对运动大鼠毒作用的免疫学机制。方法:48只SD大鼠随机分为空白对照组(SG)、单纯运动组(EG)、高污染运动组(HEG)、高污染安静组(HSG)、中污染运动组(MEG)、中污染安静组(MSG)、低污染运动组(LEG)和低污染安静组(LSG),每组6只。除SG和EG外,其余各组置于不同浓度SO2污染环境中(5mg/m3,10mg/m3,15mg/m3),运动组大鼠进行跑轮运动(8m/min,2h/天,共10天)。采用放射免疫法检测血清和肺组织IL-6、TNF-α含量。结果:EG大鼠血清和肺组织IL-6水平较SG显著升高(P<0.05);HEG、MEG、LEG大鼠血清及肺组织IL-6、TNF-α均较SG显著升高(P<0.01),且HEG、MEG大鼠IL-6和TNF-α较HSG、MSG上升更明显(P<0.01,P<0.05),LEG大鼠肺组织IL-6和TNF-α较LSG显著升高(P<0.05)。结论:SO2污染环境中运动可引起体内致炎细胞因子高表达,低浓度SO2污染下运动对肺组织的致炎作用较血清更明显。
Objective: To investigate the effects of SO2 pollution at different concentrations on serum and lung tissue IL-6 and TNF-α levels in exercise-induced rats and to reveal the effects of SO2 pollution on exercise-induced rats Toxicity of the immune mechanism. Methods: Forty eight SD rats were randomly divided into control group (SG), pure exercise group (EG), high pollution exercise group (HEG), high pollution quiet group (HSG), moderate pollution exercise group (MEG) Quiet group (MSG), low-pollution exercise group (LEG) and low-pollution quiet group (LSG), 6 in each group. In addition to SG and EG, the other groups were placed in different concentrations of SO2 contaminated environment (5mg / m3,10mg / m3, 15mg / m3), exercise group rats runner exercise (8m / min, 2h / day). The levels of IL-6 and TNF-α in serum and lung tissue were detected by radioimmunoassay. Results: The levels of IL-6 in serum and lung tissue of EG rats were significantly higher than those of SG rats (P <0.05). The levels of IL-6 and TNF-α in serum and lung tissue of HEG, MEG and LEG rats were significantly higher than those of SG <0.01). The levels of IL-6 and TNF-α in HEG and MEG rats increased more significantly than those in HSG and MSG groups (P <0.01, P <0.05) High (P <0.05). CONCLUSIONS: Exercise induced by SO2 can cause high expression of proinflammatory cytokines in vivo. The proinflammatory effect of exercise with low concentration of SO2 on lung tissue is more obvious than that of serum.