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目的 比较提取表皮下大疱病相关抗原的三种分离皮肤方法。方法 采用盐裂法、 Ｅ Ｄ Ｔ Ａ 分离法、热分离法提取真、表皮抗原，免疫印迹检测提取物中抗原的活性，结果 三种方法均可用于 Ｓ Ａ Ｂ Ｄ 抗原的提取，但热分离法在所提取抗原的敏感性上明显优于其他两种方法。结论 热分离法对抗原活性影响最小，值得优先选择和运用。
OBJECTIVE To compare three methods of skin separation for extracting epidermal bullous disease-associated antigens. Methods The antigen, antigen and immunoblotting were used to detect the activity of the antigen in the extract by the method of salt splitting, E D T A separation and thermal separation. Results Three methods were available for the extraction of S A B D antigen, but the thermal separation Method in the extracted antigen sensitivity was significantly better than the other two methods. Conclusion Thermal separation method has the least effect on antigen activity, which deserves priority selection and application.