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目的研究慢性乙型肝炎(CHB)肝活检组织α-肌动蛋白(α-SMA)的表达,探讨α-SMA在慢性乙型肝炎病毒感染中的作用及意义。方法通过RT-PCR对CHB活检组织的α-SMA进行半定量,结合肝脏组织学和免疫组化,对CHB患者的肝组织α-SMA的表达进行分析。结果在基因水平上,轻度CHB患者α-SMA/β-actin为0.126±0.032,与中度(0.323±0.099,P=0.001)和重度(0.410±0.103,P=0.000)比较差异有统计学意义;但在CHB患者中,中度和重度比较差异无统计学意义(P=0.097)。免疫组化显示:在CHB肝组织,α-SMA主要与坏死性炎症反应有关,贮脂细胞和肝窦内皮细胞表达α-SMA较强。CHB轻度组(G1/G2),α-SMA表达于坏死性炎症反应部位;CHB较重组(G3/G4),则表达于坏死区及其相邻的肝实质表达明显增加,且Kuppfer细胞明显增多。结论 HBV慢性感染状态下,α-SMA的表达上调,可能参与了与肝脏患者的CHB病理过程。
Objective To investigate the expression of α-actin (α-SMA) in chronic hepatitis B (CHB) liver biopsy and to explore the role and significance of α-SMA in chronic hepatitis B virus infection. Methods The α-SMA of CHB biopsies was semi-quantitatively determined by RT-PCR, and the expression of α-SMA in liver tissues of CHB patients was analyzed in combination with liver histology and immunohistochemistry. Results At gene level, α-SMA / β-actin in mild CHB patients was 0.126 ± 0.032, which was statistically different from moderate (0.323 ± 0.099, P = 0.001) and severe (0.410 ± 0.103, P = 0.000) However, there was no significant difference between moderate and severe CHB patients (P = 0.097). Immunohistochemistry showed that α-SMA was mainly associated with necrotic inflammatory reaction in CHB liver tissues, while α-SMA was expressed in both fat-storing and sinusoidal endothelial cells. CHB mild group (G1 / G2), α-SMA expression in the necrotic inflammatory response site; CHB heavier group (G3 / G4), the expression in the necrotic area and adjacent liver parenchyma significantly increased, and Kuppfer cells were significantly increase. Conclusion The expression of α-SMA is up-regulated in chronic HBV infected patients, which may be involved in the pathological process of CHB in patients with liver disease.