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通过不同树脂对绣球菌多糖脱色率及多糖保留率的比较,选择脱色效果好、多糖保留率高的树脂对绣球菌多糖进行脱色。实验结果表明D303和HZ-830两种型号的离子交换树脂脱色效果较好;通过单因素试验对2种树脂的脱色时间、温度、pH、上样质量浓度对脱色率影响进行研究,得出较合适的脱色工艺。实验结果表明,D303树脂最佳脱色条件为脱色温度40℃、脱色时间6 h、pH8、样品质量浓度10 mg·mL-1;HZ-830树脂最佳脱色条件为脱色温度40℃、脱色时间3 h、pH8、样品质量浓度20 mg·mL-1。
By comparing the decolorization rate of polysaccharide and the retention rate of polysaccharide by different resins, the best decoloring effect and polysaccharide retention rate were selected to decolorize the polysaccharide of Hydatid. The experimental results show that D303 and HZ-830 two types of ion exchange resin decolorization is better; by single factor test two resins decolorization time, temperature, pH, sample concentration on the decolorization rate of the impact study, Suitable decolorization process. The experimental results showed that the best decolorization conditions of D303 resin were decolorization temperature 40 ℃, decolorization time 6 h, pH8 and mass concentration 10 mg · mL-1. The best decolorization conditions of HZ-830 resin were bleaching temperature 40 ℃, decolorization time 3 h, pH8, sample concentration 20 mg · mL-1.