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本试验以普通小麦（Ｔａ）、多花黑麦草（Ｌｍ）和多年生黑麦草（Ｌｐ）细胞悬浮系来源的原生质体为材料研究了碘乙酰胺（ＩＯＡ）和若丹明（Ｒ—６Ｇ）对禾本科植物原生质休的失活效果。Ｔａ、Ｌｍ和ＬＰ原生质体经１ｍｍｏｌ／ＬＩＯＡ处理后存活率分另为３４％。１７％和０％，而对照的存活率分别为７８％、６８％和７１％。使Ｔａ和Ｌｍ原生质体全部失活的ＩＯＡ浓度需２ｍｍｏｌ／Ｌ。Ｔａ、Ｌｍ和Ｌｐ原生质体经４０μｇ／ｍｌＲ—６Ｇ处理后的存活率分别为６０％、６％和６３％，而对照的存活率分别为７０％、６１％和８７％。经４０μｇ／ｍｌＲ—６Ｇ处理后，Ｌｍ和ＬＰ原生质体植板率分别为０和１３细胞团／皿而对照的植板率分别为５９８和３９９细胞团／皿。以上结果表明，ＩＯＡ和Ｒ—６Ｇ都是有效的禾本科植物原生质体失活剂。
In this study, the protoplasts derived from cell suspending lines of common wheat (Ta), Lolium multiflorum (Lm) and perennial ryegrass (Lp) were used as materials to study the effect of iodoacetamide (IOA) and Rhodamine Gramineous plant inactivation of the protoplasm effect. The survival rate of Ta, Lm and LP protoplasts treated with 1mmol / LIOA was 34%. 17% and 0%, respectively, while the control survival rates were 78%, 68% and 71%, respectively. The concentration of IOA required to inactivate all Ta and Lm protoplasts was 2 mmol / L. Survival rates of Ta, Lm and Lp protoplasts were 60%, 6% and 63%, respectively, at 40μg / ml R-6G, while the control survival rates were 70%, 61% and 87%, respectively. After 40μg / ml R-6G treatment, the rate of planted Lm and LP protoplasts were 0 and 13 cells / dish, respectively, while the control plants were plated at 598 and 399 cells / dish, respectively. The above results show that both IOA and R-6G are effective gramineous plant protoplast inactivators.