【摘 要】
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Increasing evidence has indicated that microRNA dysregulation is closely related to the occurrence and development of cancers.Herein,we investigated the relationship between miR-144-3p and CEP55 expression.We then evaluated the association between miR-144
【机 构】
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Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine,Hunan Universi
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Increasing evidence has indicated that microRNA dysregulation is closely related to the occurrence and development of cancers.Herein,we investigated the relationship between miR-144-3p and CEP55 expression.We then evaluated the association between miR-144-3p and CEP55 expression and proliferation,invasion and apoptosis of non-small cell lung cancer (NSCLC) cells.Real-time quantitative PCR results revealed that CEP55 was over-expressed whereas miR-144-3p was under-expressed in NSCLC tissues.CCK-8 assay,wound healing assay,and flow cytometry further revealed that overexpression of miR-144-3p significantly inhibited proliferation and migration,but promoted apoptosis of A549 cells.Conversely,inhibition of miR-144-3p promoted proliferation and migration but suppressed apoptosis of H460 cells.Dual-luciferase reporter assay revealed that miR-144-3p modulated malignant properties of cancer cells by targeting CEP55.Overexpression of CEP55 partially blocked the inhibitory effect of miR-144-3p on proliferation and migration of A549 cells and induced apoptosis of A549 cells.CEP55 knockdown modulated the increase in prolifer-ation and migration and the decrease in apoptosis of H460 cells following miR-144-3p inhibition.These findings demonstrated that miR-144-3p suppresses NSCLC development by inhibiting CEP55 expression.
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