【摘 要】
：
目的 观察腺病毒介导胸苷激酶/更昔洛韦(TK/GCV)系统联合肿瘤坏死因子-α(TNF-α)对鼠膀胱癌的杀伤作用.方法 构建MB49小鼠皮下模型,随机分为对照组、TK/GCV组、TNF-α组、联
【机 构】
：
南方医科大学珠江医院器官移植科,南方医科大学南方医院泌尿外科
论文部分内容阅读
目的 观察腺病毒介导胸苷激酶/更昔洛韦(TK/GCV)系统联合肿瘤坏死因子-α(TNF-α)对鼠膀胱癌的杀伤作用.方法 构建MB49小鼠皮下模型,随机分为对照组、TK/GCV组、TNF-α组、联合治疗组.按治疗计划分组进行病毒及药物注射,实验结束测量肿瘤体积大小,进行组织病理学检查.结果 体外实验验证低浓度TNF-α联合GCV较单纯GCV对细胞的杀伤率明显增强,差异有统计学意义(P＜0.01).流式细胞仪检测,联合治疗组较单独用药组凋亡率明显提高,差异有统计学意义(P＜0.01).动物实验结束后肿瘤体积对比:TK/GCV组为(93.43±2.10) mm3、TNF-α组为(53.95±2.61)mm3、对照组为( 171.52±4.33) mm3、联合治疗组为(18.23±1.11) mm3,联合治疗组肿瘤体积较其他各组明显缩小,差异有统计学意义(P＜0.01).苏木素-伊红(HE)染色见各治疗组均出现细胞坏死凋亡,其中联合治疗组仅于周边见少量肿瘤细胞存活.结论 GCV对转染腺病毒MB49细胞及TNF-α对MB49细胞均有良好的抑制生长作用,且呈浓度依赖性；TK/GCV、TNF-α均能有效诱导凋亡,且两者具有协同作用；TK/GCV系统联合小剂量TNF-α能增强自杀基因系统对小鼠膀胱癌的治疗作用.
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