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以卷曲霉素（ＣＰＭ）产生菌卷曲链霉菌Ｓ．ｃａｐｒｅｏｌｕｓ４００６（效价为６６４０ｕ／ｍＬ）为出发菌，经ＵＶ诱变／五氯酚浓缩，获得一株效价为７９５０ｕ／ｍＬ的营养缺陷型菌株４０６－３５，再经ＥＭＳ诱变，筛选到一株抗５０００ｕ／ｍＬＣＰＭ、效价为８５１３ｕ／ｍＬ的突变株５－４１，经原生质体ＮＴＧ诱变处理，获得一株效价为９０５０ｕ／ｍＬ的高产菌Ｎ１３菌株Ｎ１３连续传代１０代，卷曲霉素效价保持稳定通过单因子试验和多因子正交试验，确定了菌株Ｎ１３的最佳发酵条件菌株Ｎ１３在最佳发酵条件下卷曲霉素效价为９４８３ｕ／ｍＬ，比出发菌Ｓ．ｃａｐｒｅｏｌｕｓ４００６在原有发酵条件的效价提高４２８％
Streptomyces coelicolor S is produced with capreomycin (CPM). capreolus 4006 (titer of 6640u / mL) as the starting strain, UV mutagenesis / pentachlorophenol concentration obtained a titer of 7950u / mL of auxotrophic strains 406-35, and then by EMS mutagenesis, screening to a Strain 5000u / mLCPM, titer of 8513u / mL mutants 5-41 NTG mutagenized by protoplast to obtain a high titer of 9050u / mL N13 strain N13 continuous passage 10 generations, The titer of valerate remained stable The optimal fermentation conditions of strain N13 were determined by single factor test and multi-factor orthogonal test. The titer of capreomycin of strain N13 was 9483u / mL, which was higher than that of the original strain S under the optimal fermentation conditions. The titer of capreolus 4006 increased by 428% in the original fermentation conditions