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目的建立直接进样-超高效液相色谱-串联质谱法测定生活饮用水中莠去津及其代谢产物的残留。方法水样经0.22μm滤膜过滤后直接进样,超高效液相色谱串联三重四级杆质谱分析;以ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱分离,乙腈-水溶液为流动相进行梯度洗脱,采用正离子扫描多反应监测(MRM)模式检测。结果莠去津及其代谢产物在各自的考察浓度范围内线性关系良好(r≥0.999 7)。方法的检出限为0.000 5μg/L~0.04μg/L,莠去津及其代谢产物在出厂水和水源水中的加标回收率分别为81.4%~104.3%、74.7%~103.7%,相对标准偏差(n=7)分别为2.3%~6.6%、3.0%~8.0%。结论该方法操作简单、快捷,灵敏度和准确度均较高,精密度较好,适用于生活饮用水中莠去津及其代谢产物残留的测定。
Objective To establish a direct injection-ultra performance liquid chromatography-tandem mass spectrometry method for the determination of atrazine and its metabolites in drinking water. Methods The samples were filtered through 0.22μm membrane and directly injected into the water. The samples were analyzed by tandem triple quadrupole mass spectrometry with ultra performance liquid chromatography (HPLC). The separation was performed on an ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) Mobile phase gradient elution, using positive ion scan multiple reaction monitoring (MRM) mode detection. Results Atrazine and its metabolites had a good linear relationship (r≥0.999 7) within the range of the investigated concentrations. The detection limits of the method were 0.000 5 μg / L ~ 0.04 μg / L. The recoveries of atrazine and its metabolites were 81.4% -104.3% and 74.7% -103.7%, respectively, in relative standard Deviations (n = 7) were 2.3% -6.6%, 3.0% -8.0% respectively. Conclusion The method is simple, rapid, sensitive and accurate, with good precision. It is suitable for the determination of atrazine and its metabolites in drinking water.