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制备了一种硼酸功能化介孔纳米材料,利用硼酸基团可以和糖肽中糖链上的顺式邻位或间位羟基发生反应形成环状二酯对糖肽进行富集,考察并比较了不同的孵育时间、孵育缓冲液、清洗方式以及洗脱液对糖肽富集的影响,优化了该材料富集糖肽的条件,建立了一种硼酸功能化介孔纳米材料用于选择性富集糖肽并用基质辅助激光解吸附电离-四极离子阱-飞行时间质谱(MALDI-QIT-MS)进行糖肽分析的方法.
A boronic acid-functionalized mesoporous nanomaterials was prepared by using boronic acid groups to react with cis- or meta-hydroxyl groups on the sugar chains in the glycopeptide to form cyclic diesters for glycopeptide enrichment. The investigation and comparison The effects of different incubation time, incubation buffer, washing method and eluant on the glycopeptide enrichment were optimized, and the conditions for the enrichment of glycopeptide were optimized. A boronic acid-functionalized mesoporous nanomaterial for selectivity Glycopeptide enrichment of glycopeptides and analysis of glycopeptides by matrix-assisted laser desorption ionization-quadrupole ion trap-time of flight mass spectrometry (MALDI-QIT-MS).