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空斑形成试验（ｐｌａｑｕｅ ｆｏｒｍｉｎｇ ｃｅｌｌｓ，ＰＦＣ）检测脂质体包裹四环素或单独四环素治疗小鼠布鲁氏菌病疗效的研究表明，ＰＦＣ作为疗效判定指标与脏器感染率有较好的吻合性．即感染率下降，ＰＦＣ增强；感染率不变，ＰＦＣ亦不变．该试验较感染率的测定方法简单、迅速、重复性好．
Plaque forming cells (PFC) test liposome-coated tetracycline or tetracycline alone treatment of brucellosis in mice showed that PFC as a curative effect indicators and organ infection rate has good agreement. The infection rate decreased, PFC increased; infection rate unchanged, PFC also unchanged. The test method for determining the infection rate is simple, rapid and reproducible.