[Objective] To study the effects of different transplanting medium ratios on the survival rate and late growth of Dendrobium officinale Kimura et Migo seedlings.[Method] 4 kinds of mediums were adopted in this study,i.e.,fine sawdust,turf:bark:wood shavings=2:4:4,turf:bark:wood shavings=3:3:4,turf:bark:fine sawdust=2:3:5.[Result] Medium formula with turf,bark and wood shavings at the ratio of 2:4:4 had stronger water and nutrient preserving capacity as well as air permeability,the survival rate and germination rate in the late growth of Dendrobium officinale Kimura et Migo seedlings in which were the highest among all the groups,besides that the stem diameter was larger and the plant height was higher.[Conclusion] Turf,bark and wood shavings medium with the ratio of 2:4:4 had the most significant impact on the growth of Dendrobium officinale Kimura et Migo,which would provide a reference for its production in the Taihu Lake basin. [Objective] To study the effects of different transplanting medium ratios on the survival rate and late growth of Dendrobium officinale Kimura et Migo seedlings. [Method] 4 kinds of mediums were adopted in this study, ie, fine sawdust, turf: bark: wood shavings = 2: 4: 4, turf: bark: wood shavings = 3: 3: 4, turf: bark: fine sawdust = 2: 3: 5. [Result] Medium formula with turf, bark and wood shavings at the ratio of 2: 4: 4 had stronger water and nutrient preserving capacity as well as air permeability, the survival rate and germination rate in the late growth of Dendrobium officinale Kimura et Migo seedlings in which the the highest among all the groups, besides that the stem diameter was larger and the plant height was higher. [Conclusion] Turf, bark and wood shavings medium with the ratio of 2: 4: 4 had the most significant impact on the growth of Dendrobium officinale Kimura et Migo, which would provide a reference for its production in the Taihu Lake basin.