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本研究利用InDel标记对新疆主栽加工番茄品种TH8号和TH17号的杂交种进行了纯度快速鉴定。从48对候选多态性丰富的InDel标记中,筛选出2对(InDel-328,InDel-78)可重复、稳定、特异的可区别各自父母本的InDel标记,分别对TH8号和TH17号杂交种进行了纯度分析,结果表明其中TH8号的平均纯度为99.29%,TH17号的平均纯度为99.65%。上述结果与大田鉴定结果高度一致。
In this study, InDel markers were used to test the purity of hybrids of main cultivated tomato varieties TH8 and TH17 in Xinjiang. Two pairs of InDel markers (InDel-328, InDel-78) were screened out from 48 pairs of candidate polymorphic InDel markers, which were repeatable, stable and specific. The purity analysis showed that the average purity of TH8 was 99.29% and that of TH17 was 99.65%. The above results are highly consistent with the field identification results.