论文部分内容阅读
目的探讨大黄酚对大鼠单侧输尿管梗阻模型(UUO)肾间质纤维化的影响。方法 27只SD大鼠随机分为假手术组、模型组、大黄酚组。用单侧输尿管梗阻术建立肾间质纤维化模型,大黄酚治疗14 d后检测血肌酐、尿素氮水平;采用免疫组织化学方法观察肾组织结缔组织生长因子(CTGF)、α-平滑肌肌动蛋白(α-SMA)、纤维连接蛋白(FN)的表达情况。结果与假手术组相比,模型组血肌酐、尿素氮的水平以及CTGF、α-SMA、FN的表达均显著增高。与模型组相比,大黄酚组血肌酐、尿素氮水平降低,CTGF、α-SMA、FN的阳性表达显著下调。结论大黄酚可能通过抑制UUO大鼠肾组织CTGF、α-SMA、FN的表达,从而缓解肾间质纤维化的发生发展。
Objective To investigate the effect of chrysophanol on renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO). Methods Twenty-seven SD rats were randomly divided into sham operation group, model group and chrysophanol group. The model of renal interstitial fibrosis was established by unilateral ureteral obstruction. Serum creatinine and urea nitrogen were measured 14 days after chrysophanol treatment. The expressions of connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), fibronectin (FN) expression. Results Compared with the sham operation group, the levels of serum creatinine, urea nitrogen and the expressions of CTGF, α-SMA and FN in model group were significantly increased. Compared with the model group, the serum creatinine and urea nitrogen in the chrysophanol group decreased, and the positive expressions of CTGF, α-SMA and FN were significantly down-regulated. Conclusion Chrysophanol may alleviate the occurrence and development of renal interstitial fibrosis by inhibiting the expression of CTGF, α-SMA and FN in the kidney of UUO rats.