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用抗青枯病花生品种远杂9102与感病品种Chico杂交,从F2起用单粒传法构建了花生重组近交系群体(RIL)F6和F7。采用354对SSR引物对重组近交系F6群体的基因组DNA鉴定,获得多态性标记45个。结合重组近交系群体F6和F7青枯病抗性鉴定结果,应用相关软件统计分析,构建了栽培种花生部分遗传连锁图。图谱总长度为603.9cM,含29个标记(28个SSR标记和1个表型标记)的8个连锁群,还有17个独立的SSR标记;获得了与青枯病抗性相关的SSR标记2个(7G02和PM137),位于该图谱的第1连锁群上,与青枯病抗性基因间的遗传距离为10.9cM和13.8cM,并且位于抗性基因的两侧,两标记间的距离为23.7cM。
A peanut variety Yuanza 9102 resistant to bacterial wilt was crossed with a susceptible cultivar Chico. The recombinant inbred line population (RIL) F6 and F7 of peanut were constructed by single grain propagation from F2. A total of 45 pairs of SSR primers were used to identify the genomic DNA of recombinant inbred line F6 population. Combined with the identification of bacterial wilt resistance of F6 and F7 in inbred lines of inbred lines, some genetic linkage maps of peanut cultivars were constructed by statistical software analysis. The total length of the map was 603.9cM, including 8 linkage groups of 29 markers (28 SSR markers and 1 phenotypic marker), and 17 independent SSR markers. SSR markers related to bacterial wilt resistance were obtained 2 (7G02 and PM137) located on the first linkage group of the map, the genetic distances to the resistance genes to bacterial wilt were 10.9cM and 13.8cM, and were located on both sides of the resistance gene. The distance between the two markers 23.7cM.