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目的将iRGD肽和PEI600连接至微泡上,制备靶向阳离子微泡。方法采用硫醇-马来酰亚胺法连接iRGD肽,通过PEI600修饰使微泡表面电荷呈现正电荷,观察制备的携iRGD肽阳离子微泡(MBiRGD)与bEnd.3内皮细胞和人MCF-7乳腺癌细胞的黏附情况,并检测MBiRGD结合质粒DNA的情况。结果制备的MBiRGD表面电荷为正电荷,能有效结合质粒DNA,并能靶向bEnd.3内皮细胞和人MCF-7乳腺癌细胞,其黏附能力明显高于非靶向微泡。结论制备的携iRGD肽阳离子微泡是一种能靶向肿瘤细胞的新型阳离子微泡。
Objective To connect iRGD peptide and PEI600 to microbubbles to prepare targeted cationic microbubbles. Methods The iRGD peptide was linked by thiol-maleimide method and the surface charges of microbubbles were positively charged by PEI600 modification. The effect of iRGD peptide cationic microbubbles (MBiRGD) and bEnd.3 endothelial cells and human MCF-7 Breast cancer cell adhesion, and to detect MBiRGD binding plasmid DNA situation. Results The surface charges of MBiRGD were positively charged, efficiently bound to plasmid DNA and targeted to bEnd.3 endothelial cells and human MCF-7 breast cancer cells. The adhesion capacity was significantly higher than that of non-targeted microbubbles. Conclusions The prepared iRGD peptide cationic microbubbles is a novel cationic microbubble that can target tumor cells.