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应用多重引物人乳头瘤病毒(HPV)6b、11、16、18型聚合酶键反应(PCR)技术检测74例新疆南疆、北疆部分地区女性下生殖道尖锐湿疣(CA)与宫颈上皮内瘤变(CIN)组织中人乳头瘤病毒DNA(HPV DNA).其中在南疆检测出CA17例,CIN Ⅲ级20例,在北疆检测出CA22例,CINⅠ～Ⅲ级2例,CINⅢ级13例.结果:总HPV DNA检出率分别为94.1%、90.0%、90.9%、86.7%.CIN与CA患者的HPVDNA检出率在地区性方面无显著性差异(P>0.05).在南、北疆患者CA组织中HPV6b、11、16、18型检出率分别为89.7%、12.8%、5.1%.CIN组织中HPV6b、11型检出率为0%,HPV16、18型检出率分别为65.7%、20%.提示CA与HPV6b、11型感染有关,CIN则与HPV16、18型密切相关.并且在南、北疆CA与CIN患者所感染的HPV亚型无地理分布差异(P>0.05).CIN组织中,HPV16、18型检出率较国内外报道高,值得重视;CA组织中,检出HPV高危型(HPV16、18型)7例,其中1例伴宫颈不典型增生Ⅰ～Ⅱ级,这些病例应继续密切随访.
Detection of female lower genital warts (CA) and cervical intraepithelial neoplasia in 74 females in parts of southern Xinjiang and northern Xinjiang using multiple primer HPV 6b, 11, 16 and 18 polymerase chain reaction (PCR) techniques Human papillomavirus DNA (HPV DNA) in neoplasia (CIN) tissue. Among them, 17 cases of CA were detected in southern Xinjiang, 20 cases of CIN III, 22 cases of CA in northern Xinjiang, 2 cases of CINI-III, and 13 cases of CIN III. Cases. Results: The detection rate of total HPV DNA was 94.1%, 90.0%, 90.9%, and 86.7%, respectively. There was no significant difference in the detection rate of HPV DNA between patients with CIN and CA (P>0.05). The detection rates of HPV6b,11,16,18 in CA patients in northern Xinjiang were 89.7%, 12.8%, and 5.1%, respectively. The detection rate of HPV6b and 11 in CIN tissues was 0%, and the detection rates of HPV 16 and 18 were respectively. At 65.7% and 20%, CA was associated with HPV 6b and 11 infection, and CIN was closely related to HPV 16 and 18, and there was no geographical difference in HPV subtypes between CA and CIN patients in the northern and southern Xinjiang. 0.05). In CIN tissues, the detection rate of HPV 16 and 18 was higher than that reported at home and abroad, and it was worthy of attention. In CA tissues, 7 HPV high-risk types (HPV16, 18) were detected, including 1 case of cervical dysplasia I. ~ Level II, these diseases We should continue to closely follow-up.