论文部分内容阅读
目的比较血管内皮细胞表面黏附分子-1(VCAM-1)及P-选择素在机械性牵张力诱导HL-60细胞向小鼠颈总动脉内皮细胞黏附中的作用。方法使用分离的小鼠颈总动脉血管在一定压力下灌流HL-60细胞,使之与血管内皮相互作用后洗去未黏附细胞,镜下统计每个视野中黏附的细胞数。首先观察不同强度牵张力刺激对于HL-60细胞向血管内皮细胞黏附的影响。再利用针对VCAM-1、P-选择素的特异性中和抗体以及非特异性IgG预处理血管条,比较VCAM-1和P-选择素在牵张力引起的HL-60细胞黏附中的差别情况。结果随着施加在颈动脉的静水压增大,HL-60细胞黏附逐渐增多,说明牵张力引起的HL-60细胞黏附是强度依赖性增加的。非特异IgG预处理血管条后,黏附的HL-60细胞数目无明显变化。然而与此对照,VCAM-1与P-选择素预处理组都导致了黏附的HL-60细胞显著减少(P<0.05),但2组间差异无统计学意义(P>0.05)。结论牵张引起的颈总动脉HL-60细胞黏附呈现压力强度依赖性关系;VCAM-1与P-选择素在机械性牵张力诱导小鼠颈总动脉HL-60细胞黏附中起一定作用。
Objective To compare the role of vascular endothelial cell adhesion molecule-1 (VCAM-1) and P-selectin in the adhesion of HL-60 cells to the common carotid artery endothelial cells induced by mechanical strain. Methods HL-60 cells were perfused with the isolated common carotid arteries of the mouse under constant pressure to wash the non-adherent cells after interaction with the vascular endothelium. The number of adherent cells in each field was counted microscopically. First observe the different strength of stretch stimulation on the adhesion of HL-60 cells to vascular endothelial cells. The specific neutralizing antibodies against VCAM-1, P-selectin and nonspecific IgG were used to pretreat the vascular strips to compare the differences of VCAM-1 and P-selectin in the stretch-induced adhesion of HL-60 cells. Results As the hydrostatic pressure applied to the carotid artery increased, the adhesion of HL-60 cells gradually increased, indicating that the tension-induced adhesion of HL-60 cells increased in a dose-dependent manner. After pretreated with non-specific IgG, there was no significant change in the number of HL-60 cells adhered. However, in contrast, both VCAM-1 and P-selectin pretreatment groups resulted in a significant decrease in adherent HL-60 cells (P <0.05), but there was no significant difference between the two groups (P> 0.05). CONCLUSION: Stretch-induced carotid artery HL-60 cell adhesion exhibits pressure-intensity-dependent relationship. VCAM-1 and P-selectin play a role in the adhesion of carotid artery HL-60 cells induced by mechanical strain.