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建立了高效液相色谱法同时测定忍冬叶中的木犀草素(1)和木犀草苷(2),并考察两者含量随采摘时间的动态变化。采用依利特Hypersil ODS C18柱,以乙腈-0.5%冰乙酸为流动相,梯度洗脱,检测波长350 nm。1和2在2.5～80 g/ml和5.25～168 g/ml浓度范围内线性良好,平均回收率为100.1%和99.9%,RSD为0.89%和1.06%。测定了不同月份采摘的忍冬叶中的1和2,结果显示1、2含量分别在11月(0.75 mg/g)和6月(6.05 mg/g)达到最高。
A high performance liquid chromatography (HPLC) was developed for the simultaneous determination of luteolin (1) and luteolin (2) in honeysuckle leaves. The dynamic changes of the contents of luteolin and luteolin were investigated. Ellite Hypersil ODS C18 column was used with a mobile phase of acetonitrile-0.5% glacial acetic acid as the mobile phase and gradient elution. The detection wavelength was 350 nm. 1 and 2 showed good linearity in the range of 2.5 ~ 80 g / ml and 5.25 ~ 168 g / ml with the average recoveries of 100.1% and 99.9% with RSDs of 0.89% and 1.06%, respectively. The results showed that the contents of 1,2 and 1,2 were the highest in November (0.75 mg / g) and June (6.05 mg / g), respectively.