氨基胍和维生素C对糖尿病肾病模型大鼠血清层粘连蛋白的影响

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目的:研究氨基胍、维生素C对糖尿病肾病模型大鼠血清层粘连蛋白的影响。方法:将大鼠随机分为正常对照组、糖尿病组和维生素C、氨基胍、维生素C+氨基胍治疗组;除正常对照组外,其余4组经腹腔注射链脲佐菌素建立1型糖尿病模型,造模成功后各组给予相应药物治疗16wk,观察并测定治疗期间及治疗后大鼠的一般状况、血糖、糖化血红蛋白、层粘连蛋白、尿素氮、血肌酐变化及24h尿蛋白排泄率。结果:造模后4组大鼠均出现血清层粘连蛋白升高和糖尿病性肾病;氨基胍、维生素C对血糖无影响,但能改善基本状况,二者合用比单用能显著降低糖尿病大鼠的血清层粘连蛋白含量、尿素氮、血肌酐及24h尿蛋白排泄率。结论:氨基胍、维生素C无降糖作用,但合用后可降低血清层粘连蛋白含量,保护肾功能,具有协同作用。 Objective: To study the effects of aminoguanidine and vitamin C on serum laminin in diabetic nephropathy model rats. Methods: The rats were randomly divided into normal control group, diabetic group and vitamin C, aminoguanidine, vitamin C + aminoguanidine treatment group; in addition to the normal control group, the other 4 groups were intraperitoneal injection of streptozotocin to establish type 1 diabetes model After the model was established, the rats in each group were given the corresponding drugs for 16 weeks. The general condition, blood glucose, glycosylated hemoglobin, laminin, urea nitrogen, serum creatinine and urinary protein excretion rate during the treatment and after treatment were observed and measured. RESULTS: After modeling, serum laminin and diabetic nephropathy appeared in all the four groups. Aminoguanidine and vitamin C had no effect on blood glucose, but could improve the basic condition. The combination of the two agents could significantly reduce diabetic rats Serum laminin, urea nitrogen, serum creatinine and urinary protein excretion rate. CONCLUSION: Aminoguanidine and vitamin C have no hypoglycemic effect, but synergistic effect can be achieved by reducing serum laminin level and protecting renal function.
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