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目的观察雌激素对去势大鼠肝脂肪变作用,探讨雌激素肝保护作用及可能机制。方法 6周SD雌性大鼠随机分为3组:假手术组、手术组和雌二醇组,术后1周,雌二醇组大鼠每天灌胃戊酸雌二醇(1 mg/kg)水溶液,其他组大鼠给予蒸馏水,连续12周。进行胰岛素耐量试验,采用酶联免疫法测定肝组织中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和单核细胞趋化蛋白-1(MCP-1)含量。苏木素-伊红染色观察肝脂肪变性和炎症状况。结果与假手术组比较,手术组大鼠体重、肝重、内脏脂肪重量[分别为(439.9±53.1)、(12.6±1.9)、(29.6±12.1)g]及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平[分别为(57.8±5.4)、(128.4±10.1)U/L]明显升高(P<0.05);与手术组比较,雌二醇组大鼠体重、肝重、内脏脂肪重量[分别为(387.3±40.7)、(10.3±2.1)、(24.1±6.5)g]及血清ALT、AST水平[分别为(44.5±4.8)、(98.2±9.6)U/L]明显降低(P<0.05)。手术组大鼠肝小叶结构模糊,肝索排列紊乱,有广泛的脂滴形成,小叶内及汇管区可见炎症细胞浸润;雌二醇组大鼠肝小叶结构仍欠清晰,脂滴明显减少,且脂滴明显变小;与手术组比较,雌二醇组大鼠肝脂肪变程度及小叶内炎症程度显著减轻。与假手术组比较,手术组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(186.3±17.2)、(187.6±26.5)、(112.3±22.4)pg/m L]明显升高(P<0.05);与手术组比较,雌二醇组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(132.6±8.6)、(110.8±21.2)、(89.1±10.8)pg/m L]明显降低(P<0.05)。结论雌激素可减轻去势大鼠肝脂肪变性程度,其机制可能与降低肝组织炎性因子表达有关。
Objective To observe the effect of estrogen on hepatic steatosis in ovariectomized rats and to explore the protective effect of estrogen on liver and its possible mechanism. Methods SD female rats were randomly divided into 3 groups: sham operation group, operation group and estradiol group. One week after operation, estradiol valerate (1 mg / kg) The other groups were given distilled water for 12 weeks. The levels of TNF-α, IL-6 and MCP-1 in liver tissue were determined by enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin staining for hepatic steatosis and inflammatory conditions. Results Compared with the sham operation group, body weight, liver weight, visceral fat weight [(439.9 ± 53.1), (12.6 ± 1.9), (29.6 ± 12.1) g] and serum ALT, The levels of aspartate aminotransferase (AST) were significantly higher in the estradiol group (57.8 ± 5.4 and (128.4 ± 10.1) U / L, P <0.05) Fat weight [(387.3 ± 40.7), (10.3 ± 2.1), (24.1 ± 6.5) g] and serum ALT and AST levels (44.5 ± 4.8 and 98.2 ± 9.6 U / L, respectively) (P <0.05). In the operation group, the hepatic lobule structure was obscured, the hepatic cord was disordered, a wide range of lipid droplets were formed, and inflammatory cell infiltration was seen in the lobular and portal area. The structure of hepatic lobule in the estradiol group was still not clear and the lipid drop was significantly reduced Lipid drop significantly smaller; Compared with the operation group, the level of hepatic steatosis and the severity of intrahepatic lobule inflammation in the estradiol group were significantly reduced. Compared with the sham group, the levels of TNF-α, IL-6 and MCP-1 in the liver tissue of the operation group were (186.3 ± 17.2), (187.6 ± 26.5) and (112.3 ± 22.4) pg / m (P <0.05). Compared with the operation group, the contents of TNF-α, IL-6 and MCP-1 in the liver homogenate of estradiol group were (132.6 ± 8.6) and ± 21.2), (89.1 ± 10.8) pg / m L] (P <0.05). Conclusion Estrogen can reduce hepatic steatosis in ovariectomized rats, and its mechanism may be related to the decrease of expression of inflammatory cytokines in liver tissues.