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目的 :研究树黑质至上丘的γ 氨基丁酸 (γ aminobutyricacid ,GABA)能和多巴胺 (Depamine ,DOPA)能阳性纤维投射。方法 :采用荧光金 (Fluoro Gold ,FG)逆行追踪分别与GABA和DOPA免疫组化相结合技术。结果 :在树双侧黑质的致密部、网状部和外侧部均观察到FG逆行标记细胞 ;双侧黑质的网状部除向上丘发出GABA能纤维投射外 ,同侧黑质网状部还向上丘发出DOPA能纤维投射。此外 ,在同侧黑质的致密部 ,还观察到少量的向上丘投射的DOPA能神经元。结论 :黑质向上丘的GABA和DOPA能阳性纤维投射为阐明黑质的生理功能提供形态学依据。
Objective: To study the effects of γ aminobutyric acid (GABA) and dopamine (DOPA) positive fibers on the substantia nigra and superior colliculus in tree shrews. Methods: Fluoro Gold (FG) retrograde tracing technique was used to detect GABA and DOPA immunohistochemistry respectively. RESULTS: FG retrograde marker cells were observed in the dense, reticular and lateral portions of the substantia nigra on both sides of the tree shrew. In addition to the projection of GABA fibers toward the superior colliculus, The Ministry also issued to the uphill DOPA fiber projection. In addition, a small amount of DOPA-capable neurons projecting to the superior colliculus were also observed in the ipsilateral substantia nigra compact part. CONCLUSIONS: The projection of GABA and DOPA positive fibers from the substantia nigra to the superior colliculus provides a morphological basis for elucidating the physiological function of the substantia nigra.