Tomato has emerged as an emblematic model plants for fleshy fruit research and tomato fruit ripening is a complex and highly coordinated developmental process.The many physiology and biochemical processes associated with tomato fruit ripening require changes in the expression of hundreds to thousands of genes.Gene expression is regulated by transcriptional and post-transcriptional pathways,one of the recently discovered mechanisms in plants was small RNAs mediated gene silencing at post-transcriptional(PTGS) level.Intriguingly,several mi RNAs and endogenous si RNAs were revealed to be involved in the fruit ripening process which opened a new avenue in the field of fleshy fruit biology.This review compiled the most recent advances made in deciphering the regulation functions of mi RNAs and si RNAs in tomato fruit ripening.It also emphasized the new perspectives now possible in the small RNAs regulation research in tomato fruit ripening and senescence. Tomato has emerned as an emblematic model plants for fleshy fruit research and tomato fruit ripening is a complex and highly coordinated developmental process. The many physiology and biochemical processes associated with tomato fruit ripening require changes in the expression of hundreds to thousands of genes. Gene expression is regulated by transcriptional and post-transcriptional pathways, one of the newly discovered mechanisms in plants was small RNAs mediated gene silencing at post-transcriptional (PTGS) level. Intriguingly, several mi RNAs and endogenous si RNAs were revealed to be involved in the fruit ripening process which opened a new avenue in the field of fleshy fruit biology. This review compiled the most recent advances made in deciphering the regulatory functions of mi RNAs and si RNAs in tomato fruit ripening. Also also emphasized the new perspectives now possible in the small RNAs regulation research in tomato fruit ripening and senescence.