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用薄层扫描仪进行定量测定的方法有外标法(ext-ernal standard method)和内标法(internal standardmethod)两法,而外标法更为常用。现结合岛津薄层扫描仪操作说明书详细叙述此两法。外标法例如在1块10×10cm 的硅胶 G 板上,按“薄层扫描法”一文[见本刊1987;(4):234]的方法和规定点样。标准品配成约1mg/ml 溶液,配在1ml 容量瓶中。点1,2,3,4和5μl各2个点,共10个点。为了避免不同的定容毛细管之间的误差,用同一根1μl定容毛细
The methods of quantitative determination using a thin-layer scanner include the ext-ernal standard method and the internal standard method, and the external standard method is more commonly used. Now combined with the Shimadzu thin-film scanner operating manual detailing these two methods. The external standard method is, for example, spotting on a 10 × 10 cm silica gel G plate according to the method and regulation of “Thin Layer Scanning Method” (see Journal 1987; (4): 234). Standard dubbed about 1mg / ml solution, equipped with 1ml volumetric flask. Points 1, 2, 3, 4 and 5 μl each 2 points for a total of 10 points. In order to avoid the discrepancy between the capillaries of the capillary, use the same 1μl capillaries