观察外源野生型p5 3基因在肝癌基因治疗方面的可行性。方法 :将载有人野生型 p5 3-cDNA的真核表达质粒pcDNA3 - p5 3,用阳离子脂质体介导转染人肝癌细胞系HepG3B细胞 ,用流式细胞仪检测pcDNA3-p5 3对HepG3B细胞生长的影响。结果 :通过观察细胞生长曲线与流式细胞仪检测细胞周期和细胞的凋亡指数发现 ,HepG3B细胞生长受到明显的抑制。结论 :脂质体介导的p5 3基因可在HepG3B细胞中表达 ,且明显抑制该细胞的生长。 To observe the feasibility of exogenous wild-type p53 gene in hepatocellular carcinoma gene therapy. Methods: The eukaryotic expression plasmid pcDNA3 - p5 3 carrying human wild type p5 3 - cDNA was transfected into HepG3B cells by cationic liposome. The expression of pcDNA3 - p5 3 was detected by flow cytometry in HepG3B cells The impact of growth. Results: The cell growth curve and flow cytometry were used to detect cell cycle and apoptosis index. The growth of HepG3B cells was significantly inhibited. Conclusion: The liposome-mediated gene p5 3 can be expressed in HepG3B cells and significantly inhibit the growth of the cells.