目的:研究CD40和CD40L在大鼠炎症性肠炎(IBD)外周血和结肠组织单-核细胞(MC)中的表达与免疫功能紊乱的关系。方法:利用流式细胞仪对20例炎症性肠炎大鼠和20例正常对照组外周血、结肠组织MC上CD3、CD4、CD8、CD19的表达进行检测;CD40L在CD4+T和CD8+T细胞上的表达及CD40在CD19+B细胞上的表达进行检测。结果:IBD大鼠外周血、结肠组织CD3+、CD3+CD4+、CD19+细胞较正常对照组显著增高,CD3+CD8+细胞较正常对照组显著降低;IBD大鼠外周血、结肠组织CD4+T细胞和CD8+T细胞上的CD40L、D19+B细胞上CD40的表达都较对照组显著增高,且结肠组织上的表达又比外周血高。结论:CD40-CD40L途径在IBD免疫功能紊乱中可能起了重要作用。 AIM: To investigate the relationship between the expression of CD40 and CD40L in peripheral blood and colon mononuclear cells (MC) in rats with inflammatory bowel disease (IBD) and immune dysfunction. Methods: The expression of CD3, CD4, CD8 and CD19 in peripheral blood and colonic tissue of 20 inflammatory bowel disease rats and 20 normal controls were detected by flow cytometry. The expressions of CD40L in CD4 + T and CD8 + T cells And the expression of CD40 on CD19 + B cells. Results: The levels of CD3 +, CD3 +, CD4 + and CD19 + in peripheral blood and colonic tissue of IBD rats were significantly higher than those of normal controls, while the levels of CD3 + CD8 + cells in IBD rats were significantly lower than those in normal controls. CD4 + T cells and CD8 + T cells CD40L, D19 + B cells CD40 expression was significantly higher than the control group, and colon tissue expression higher than peripheral blood. Conclusion: The CD40-CD40L pathway may play an important role in the immunological dysfunction of IBD.