为合成含有α-亚麻酸(α-LNA)的多烯磷脂酰胆碱,我们用高纯度蛋黄磷脂和含亚麻酸油脂在定向脂肪酶的催化下进行了酯交换反应研究,考察了亚麻酸分子形式、反应体系、含水量、底物浓度比及原料亚麻酸纯度对结合率的影响,结果表明,以正己烷作反应介质,加入较高纯度的亚麻酸乙酯(含量72.7%),底物浓度比1∶3(g/g),加水量1.0%,脂肪酶20%,在45℃恒温水浴下,搅拌反应12h.卵磷脂的亚麻酸结合率可达20%以上. To synthesize polyene phosphatidylcholine containing α-linolenic acid (α-LNA), we studied the transesterification of high-purity egg-yolk lecithin and linolenic acid-containing lipase under directional lipase catalysis. The reaction system, the water content, the concentration ratio of the substrate and the purity of the linoleic acid were investigated. The results showed that ethyl n-octanoate (72.7% content) and n-hexane The concentration of lecithin was 1:20 (g / g), the amount of water was 1.0% and the lipase was 20%, and the reaction was stirred for 12h under the constant temperature water bath at 45 ℃.