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采用表达单价Cry1Ac毒素的转Bt基因棉棉叶喂饲法比较了棉铃虫Helicoverpa armigera(Hbner)抗、感亲本及正反交后代在Bt棉上5d的发育速率,确定棉铃虫在Bt棉上5d发育到2龄中期以上、体重≥0.6mg的个体作为判别抗性纯合子的标准。2006年采用F1代法在室内用Bt棉叶喂饲法检测河北省邱县Bt棉田棉铃虫对Bt棉的抗性等位基因频率。结果表明,127头田间雄虫中24头携带抗性基因,估测抗性等位基因频率为0.94(95%CI:0.044～0.145),该值为在国内首次检测到的高抗性等位基因频率。该地区长期大面积种植转单价Bt棉和缺少非Bt棉作为有效庇护区导致田间抗性快速进化,需要尽快采取有效的抗性治理策略。本文还讨论了影响大田产生抗性的因素以及田间存在的抗性风险等。
The transgenic cotton bollworm, Helicoverpa armigera (Hbner) resistant and susceptible parents, and the progenies of reciprocal crosses were used to determine the developmental rate of Bt cotton on Bt cotton On the development of 5d to 2 years old metaphase, weight ≥ 0.6mg individuals as discriminating resistance homozygosity criteria. In 2006, F1 generation method was used to detect the frequency of resistance alleles of Bt cotton to Bt cotton in Bt cotton field in Qiu County, Hebei Province using Bt cotton leaf feeding method indoors. The results showed that 24 heads of resistance genes were detected in 127 field male adults and the frequency of resistance alleles was 0.94 (95% CI: 0.044-0.145), which was the first test of high resistance in China Gene frequency. The long-term large-scale conversion of Bt cotton into monoliths in the region and the lack of non-Bt cotton as effective sanctuaries led to a rapid evolution of resistance in the field and the need for an effective resistance management strategy as soon as possible. This article also discusses the factors that affect the resistance in the field as well as the resistance risks existing in the field.